nod like receptor protein 3 (Cell Signaling Technology Inc)
Structured Review
Nod Like Receptor Protein 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nod+like+receptor+protein+3/NLRP3+Rabbit+mAb/pmc12765096-131-57-63
Average 98 stars, based on 1666 article reviews
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Nucleic Acid Electrophoresis:Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), Transferring:Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), Membrane:Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), Article Title: Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis. Article Snippet: Accumulating evidences have suggested that extracellular vesicles (EVs) are crucial players in the pathogenesis of ischemic brain injury.. This study was designed to explore the specific functions of M2 phenotype microglia-derived EVs in ischemic brain injury progression.. The expression of microRNA-135a-5p (miR-135a-5p) in M2 microglia-derived EVs was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), followed by the identification of expression relationship among miR-135a-5p, thioredoxin-interacting protein (TXNIP), and nod-like receptor protein 3 (NLRP3) by dual luciferase reporter gene assay. Article Title: Therapeutic effects of CD133 + Exosomes on liver function after stroke in type 2 diabetic mice Article Snippet: The gel was placed in a tank and run for approximately 90 min at 120 V. The gel was subsequently transferred to a nitrocellulose membrane using the iBlot transfer system (Thermo Fisher Scientific, Waltham, MA, USA). .. The membrane was blocked in 2% I-Block (Thermo Fisher Scientific, Waltham, MA, USA) in 1 × TBS-T for 1 h, and then primary antibodies against either β-actin (1:10,000, Abcam, Waltham, MA, USA, cat# ab6276), serum amyloid A (SAA, 1:1000, Abcam, Waltham, MA, USA, cat # ab199030), Article Title: Macrophage κ-opioid receptor inhibits hypoxic pulmonary hypertension progression and right heart dysfunction via an SCD1-dependent anti-inflammatory response Article Snippet: The protein concentration was determined with a BCA kit (Shanghai Epizyme Biomedical Technology, China), followed by separation of the proteins on a 10% SDS‒PAGE gel (Shanghai Epizyme Biomedical Technology). .. After SDS‒PAGE, the protein bands were transferred onto nitrocellulose membranes and blocked with Tris-buffered saline containing 0.1% Tween 20 and 5% bovine serum albumin for 1 h. The membrane was subsequently incubated overnight at 4 °C with primary antibodies (ratio: 1:1000) against κ-OR (57,282, Signalway Antibody, USA), stearoyl coenzyme desaturase 1 (SCD1, 2794S, Cell Signaling Technology, USA), Incubation:Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), Article Title: Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis. Article Snippet: Accumulating evidences have suggested that extracellular vesicles (EVs) are crucial players in the pathogenesis of ischemic brain injury.. This study was designed to explore the specific functions of M2 phenotype microglia-derived EVs in ischemic brain injury progression.. The expression of microRNA-135a-5p (miR-135a-5p) in M2 microglia-derived EVs was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), followed by the identification of expression relationship among miR-135a-5p, thioredoxin-interacting protein (TXNIP), and nod-like receptor protein 3 (NLRP3) by dual luciferase reporter gene assay. Article Title: Macrophage κ-opioid receptor inhibits hypoxic pulmonary hypertension progression and right heart dysfunction via an SCD1-dependent anti-inflammatory response Article Snippet: The protein concentration was determined with a BCA kit (Shanghai Epizyme Biomedical Technology, China), followed by separation of the proteins on a 10% SDS‒PAGE gel (Shanghai Epizyme Biomedical Technology). .. After SDS‒PAGE, the protein bands were transferred onto nitrocellulose membranes and blocked with Tris-buffered saline containing 0.1% Tween 20 and 5% bovine serum albumin for 1 h. The membrane was subsequently incubated overnight at 4 °C with primary antibodies (ratio: 1:1000) against κ-OR (57,282, Signalway Antibody, USA), stearoyl coenzyme desaturase 1 (SCD1, 2794S, Cell Signaling Technology, USA), Activity Assay:Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), Saline:Article Title: Macrophage κ-opioid receptor inhibits hypoxic pulmonary hypertension progression and right heart dysfunction via an SCD1-dependent anti-inflammatory response Article Snippet: The protein concentration was determined with a BCA kit (Shanghai Epizyme Biomedical Technology, China), followed by separation of the proteins on a 10% SDS‒PAGE gel (Shanghai Epizyme Biomedical Technology). .. After SDS‒PAGE, the protein bands were transferred onto nitrocellulose membranes and blocked with Tris-buffered saline containing 0.1% Tween 20 and 5% bovine serum albumin for 1 h. The membrane was subsequently incubated overnight at 4 °C with primary antibodies (ratio: 1:1000) against κ-OR (57,282, Signalway Antibody, USA), stearoyl coenzyme desaturase 1 (SCD1, 2794S, Cell Signaling Technology, USA), |

