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nod like receptor protein 3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc nod like receptor protein 3
    Nod Like Receptor Protein 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nod+like+receptor+protein+3/NLRP3+Rabbit+mAb/pmc12765096-131-57-63
    Average 98 stars, based on 1666 article reviews
    nod like receptor protein 3 - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice
    Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), NOD-like receptor protein 3 (NLRP3, 1:1000, CST), apoptosis-associated speck like protein (ASC, 1:1000, ZEN BIO), postsynaptic density protein 95 (PSD95, 1:1000, CST), activity-regulated cytoskeletal protein (Arc, 1:1000, Synaptic Systems), and β-actin (1:1000, CST). ..

    Transferring:

    Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice
    Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), NOD-like receptor protein 3 (NLRP3, 1:1000, CST), apoptosis-associated speck like protein (ASC, 1:1000, ZEN BIO), postsynaptic density protein 95 (PSD95, 1:1000, CST), activity-regulated cytoskeletal protein (Arc, 1:1000, Synaptic Systems), and β-actin (1:1000, CST). ..

    Membrane:

    Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice
    Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), NOD-like receptor protein 3 (NLRP3, 1:1000, CST), apoptosis-associated speck like protein (ASC, 1:1000, ZEN BIO), postsynaptic density protein 95 (PSD95, 1:1000, CST), activity-regulated cytoskeletal protein (Arc, 1:1000, Synaptic Systems), and β-actin (1:1000, CST). ..

    Article Title: Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis.
    Article Snippet: Accumulating evidences have suggested that extracellular vesicles (EVs) are crucial players in the pathogenesis of ischemic brain injury.. This study was designed to explore the specific functions of M2 phenotype microglia-derived EVs in ischemic brain injury progression.. The expression of microRNA-135a-5p (miR-135a-5p) in M2 microglia-derived EVs was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), followed by the identification of expression relationship among miR-135a-5p, thioredoxin-interacting protein (TXNIP), and nod-like receptor protein 3 (NLRP3) by dual luciferase reporter gene assay.

    Article Title: Therapeutic effects of CD133 + Exosomes on liver function after stroke in type 2 diabetic mice
    Article Snippet: The gel was placed in a tank and run for approximately 90 min at 120 V. The gel was subsequently transferred to a nitrocellulose membrane using the iBlot transfer system (Thermo Fisher Scientific, Waltham, MA, USA). .. The membrane was blocked in 2% I-Block (Thermo Fisher Scientific, Waltham, MA, USA) in 1 × TBS-T for 1 h, and then primary antibodies against either β-actin (1:10,000, Abcam, Waltham, MA, USA, cat# ab6276), serum amyloid A (SAA, 1:1000, Abcam, Waltham, MA, USA, cat # ab199030), NOD-like receptor protein 3 (NLRP3, 1:1000, Cell Signaling, Danvers, MA, USA, cat# 15101), Interleukin-1β (IL-1β, 1:1000, Abcam, Waltham, MA, USA, cat# ab2105), IL-6 (1:1000, Fisher Scientific, Hampton, NH, USA, cat# 700480), Tumor necrosis factor α (TNFα, 1:1000, Abbiotec, Escondido, CA, USA, cat# 250844), Toll like receptor 4 (TLR4, 1:500, Santa Cruz, Dallas, TX, USA, cat# sc-10741) were used. .. Secondary antibodies (anti-mouse, Jackson ImmunoResearch, West Grove, PA, USA) were added at 1:3,000 dilution in 2% I-Block in 1 × TBS-T at room temperature for 1 h. The membranes were washed with 1 × TBS-T, and then Luminol Reagent (Santa Cruz, Dallas, TX, USA) was added.

    Article Title: Macrophage κ-opioid receptor inhibits hypoxic pulmonary hypertension progression and right heart dysfunction via an SCD1-dependent anti-inflammatory response
    Article Snippet: The protein concentration was determined with a BCA kit (Shanghai Epizyme Biomedical Technology, China), followed by separation of the proteins on a 10% SDS‒PAGE gel (Shanghai Epizyme Biomedical Technology). .. After SDS‒PAGE, the protein bands were transferred onto nitrocellulose membranes and blocked with Tris-buffered saline containing 0.1% Tween 20 and 5% bovine serum albumin for 1 h. The membrane was subsequently incubated overnight at 4 °C with primary antibodies (ratio: 1:1000) against κ-OR (57,282, Signalway Antibody, USA), stearoyl coenzyme desaturase 1 (SCD1, 2794S, Cell Signaling Technology, USA), NOD-like receptor protein 3 (NLRP3, 15,101, Cell Signaling Technology), IL-1β (ab283818, Abcam, USA), TNF-α (44,074, Signalway Antibody), IL-6 (ab229381, Abcam), smooth muscle 22 alpha (SM22α, Cat No.60213-1-Ig, Proteintech), and β-actin (Cat No.66009-1-Ig, Proteintech). .. Horseradish peroxidase-labeled goat anti-mouse IgG (A0216, Beyotime Biotechnology, China) and goat anti-rabbit IgG (A0208, Beyotime Biotechnology) were then applied at 37 °C for 1 h at a dilution ratio of 1:5000.

    Incubation:

    Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice
    Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), NOD-like receptor protein 3 (NLRP3, 1:1000, CST), apoptosis-associated speck like protein (ASC, 1:1000, ZEN BIO), postsynaptic density protein 95 (PSD95, 1:1000, CST), activity-regulated cytoskeletal protein (Arc, 1:1000, Synaptic Systems), and β-actin (1:1000, CST). ..

    Article Title: Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis.
    Article Snippet: Accumulating evidences have suggested that extracellular vesicles (EVs) are crucial players in the pathogenesis of ischemic brain injury.. This study was designed to explore the specific functions of M2 phenotype microglia-derived EVs in ischemic brain injury progression.. The expression of microRNA-135a-5p (miR-135a-5p) in M2 microglia-derived EVs was determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), followed by the identification of expression relationship among miR-135a-5p, thioredoxin-interacting protein (TXNIP), and nod-like receptor protein 3 (NLRP3) by dual luciferase reporter gene assay.

    Article Title: Macrophage κ-opioid receptor inhibits hypoxic pulmonary hypertension progression and right heart dysfunction via an SCD1-dependent anti-inflammatory response
    Article Snippet: The protein concentration was determined with a BCA kit (Shanghai Epizyme Biomedical Technology, China), followed by separation of the proteins on a 10% SDS‒PAGE gel (Shanghai Epizyme Biomedical Technology). .. After SDS‒PAGE, the protein bands were transferred onto nitrocellulose membranes and blocked with Tris-buffered saline containing 0.1% Tween 20 and 5% bovine serum albumin for 1 h. The membrane was subsequently incubated overnight at 4 °C with primary antibodies (ratio: 1:1000) against κ-OR (57,282, Signalway Antibody, USA), stearoyl coenzyme desaturase 1 (SCD1, 2794S, Cell Signaling Technology, USA), NOD-like receptor protein 3 (NLRP3, 15,101, Cell Signaling Technology), IL-1β (ab283818, Abcam, USA), TNF-α (44,074, Signalway Antibody), IL-6 (ab229381, Abcam), smooth muscle 22 alpha (SM22α, Cat No.60213-1-Ig, Proteintech), and β-actin (Cat No.66009-1-Ig, Proteintech). .. Horseradish peroxidase-labeled goat anti-mouse IgG (A0216, Beyotime Biotechnology, China) and goat anti-rabbit IgG (A0208, Beyotime Biotechnology) were then applied at 37 °C for 1 h at a dilution ratio of 1:5000.

    Activity Assay:

    Article Title: Use of Steaming Process to Improve Biochemical Activity of Polygonatum sibiricum Polysaccharides against D-Galactose-Induced Memory Impairment in Mice
    Article Snippet: .. Thereafter, protein samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 75 V for 15 min, followed by 115 V for 75 min before transferring at 300 mA onto a nitrocellulose membrane for 2 h. Membranes were blocked with 5% skim milk (dissolved in PBS containing 0.1% Tween-20) for 2 h at room temperature, washed three times with PBS for 10 min each, then incubated at 4 °C overnight in primary antibody against the α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) subunits GluA1 (1:1000, CST) and GluA2 (1:1000, CST), the N-methyl-D-aspartate receptor (NMDAR) subunits GluN2A (1:1000, CST), GluN2B (1:1000, CST), and p-GluN2B (1:1000, CST), nuclear factor-erythroid factor 2-related factor 2 (Nrf2, 1:1000, ZEN BIO), heme oxygenase-1 (HO-1, 1:1000, ZEN BIO), NOD-like receptor protein 3 (NLRP3, 1:1000, CST), apoptosis-associated speck like protein (ASC, 1:1000, ZEN BIO), postsynaptic density protein 95 (PSD95, 1:1000, CST), activity-regulated cytoskeletal protein (Arc, 1:1000, Synaptic Systems), and β-actin (1:1000, CST). ..

    Saline:

    Article Title: Macrophage κ-opioid receptor inhibits hypoxic pulmonary hypertension progression and right heart dysfunction via an SCD1-dependent anti-inflammatory response
    Article Snippet: The protein concentration was determined with a BCA kit (Shanghai Epizyme Biomedical Technology, China), followed by separation of the proteins on a 10% SDS‒PAGE gel (Shanghai Epizyme Biomedical Technology). .. After SDS‒PAGE, the protein bands were transferred onto nitrocellulose membranes and blocked with Tris-buffered saline containing 0.1% Tween 20 and 5% bovine serum albumin for 1 h. The membrane was subsequently incubated overnight at 4 °C with primary antibodies (ratio: 1:1000) against κ-OR (57,282, Signalway Antibody, USA), stearoyl coenzyme desaturase 1 (SCD1, 2794S, Cell Signaling Technology, USA), NOD-like receptor protein 3 (NLRP3, 15,101, Cell Signaling Technology), IL-1β (ab283818, Abcam, USA), TNF-α (44,074, Signalway Antibody), IL-6 (ab229381, Abcam), smooth muscle 22 alpha (SM22α, Cat No.60213-1-Ig, Proteintech), and β-actin (Cat No.66009-1-Ig, Proteintech). .. Horseradish peroxidase-labeled goat anti-mouse IgG (A0216, Beyotime Biotechnology, China) and goat anti-rabbit IgG (A0208, Beyotime Biotechnology) were then applied at 37 °C for 1 h at a dilution ratio of 1:5000.



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    (A) Through flow cytometry, the percentages of M1 macrophages (F4/80 + CD11b + CD86 + cells) and M2 macrophages (F4/80 + CD11b + CD206 + cells) in the livers of WT and ob/ob mice were determined. (B-D) Western blotting was employed to analyze CD206, CD86, CD80, <t>NLRP3,</t> and IL-1β in the liver of WT and ob/ob mice. The data are exhibited as the mean ± SEM in each group (n = 3-6).* p < 0.05, ** p < 0.01, n.s. not significant.
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    Genetic or pharmaceutical inhibition of PARP-1 increases apoptosis in primary cultured chondrocytes. a MTT assay was performed to evaluate the effect of CHX and 3-AB on chondrocyte viability ( n = 8). Chondrocytes were treated with CHX (25–100 µM), and 3-AB (100 µM) for 24 h. TUNEL assay was performed in primary cultured chondrocytes treated with CHX (50 µM) and 3-AB (100 µM) ( b ), with quantification results presented ( c , n = 6). Western blot analysis was also performed ( d ). Flow cytometry assay with PI/Annexin V was performed in primary cultured chondrocytes treated with CHX (50 µM) and 3-AB (100 µM) ( e ), with quantification results presented ( f , n = 8). Also, PI/Annexin V assay was performed in primary cultured chondrocytes from WT and PARP-1 KO treated with CHX ( g ), and quantification results were shown ( h , n = 8). TUNEL assay was performed in primary cultured chondrocytes from WT and PARP-1 KO treated with CHX ( i ), and quantification results were shown ( j , n = 6). Protein levels of PAPR-1, PAR, and c-caspase-3 were analyzed by Western blotting ( k ). Inflammasome-related factors, including NLRP3, caspase-1, and IL-1β, were also assessed ( l ). Western blotting data were collected from biological triplicate. All values, derived from the two-tailed Student’s t-test, are expressed as the mean ± standard error of the mean. Abbreviations: MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CHX, cycloheximide; 3-AB, 3-aminobenzamide; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; WT, wild-type; KO, knockout; PARP-1, poly (ADP-ribose) polymerase-1; PAR, poly ADP-ribose; c-caspase-3, cleaved caspase-3; NLRP3, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; IL-1β, interleukin-1 beta

    Journal: Arthritis Research & Therapy

    Article Title: PARP-1 prevents osteoarthritis pathogenesis by inhibiting apoptosis in chondrocytes: an animal study

    doi: 10.1186/s13075-026-03728-7

    Figure Lengend Snippet: Genetic or pharmaceutical inhibition of PARP-1 increases apoptosis in primary cultured chondrocytes. a MTT assay was performed to evaluate the effect of CHX and 3-AB on chondrocyte viability ( n = 8). Chondrocytes were treated with CHX (25–100 µM), and 3-AB (100 µM) for 24 h. TUNEL assay was performed in primary cultured chondrocytes treated with CHX (50 µM) and 3-AB (100 µM) ( b ), with quantification results presented ( c , n = 6). Western blot analysis was also performed ( d ). Flow cytometry assay with PI/Annexin V was performed in primary cultured chondrocytes treated with CHX (50 µM) and 3-AB (100 µM) ( e ), with quantification results presented ( f , n = 8). Also, PI/Annexin V assay was performed in primary cultured chondrocytes from WT and PARP-1 KO treated with CHX ( g ), and quantification results were shown ( h , n = 8). TUNEL assay was performed in primary cultured chondrocytes from WT and PARP-1 KO treated with CHX ( i ), and quantification results were shown ( j , n = 6). Protein levels of PAPR-1, PAR, and c-caspase-3 were analyzed by Western blotting ( k ). Inflammasome-related factors, including NLRP3, caspase-1, and IL-1β, were also assessed ( l ). Western blotting data were collected from biological triplicate. All values, derived from the two-tailed Student’s t-test, are expressed as the mean ± standard error of the mean. Abbreviations: MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; CHX, cycloheximide; 3-AB, 3-aminobenzamide; TUNEL, terminal deoxynucleotidyl transferase dUTP nick end labeling; WT, wild-type; KO, knockout; PARP-1, poly (ADP-ribose) polymerase-1; PAR, poly ADP-ribose; c-caspase-3, cleaved caspase-3; NLRP3, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3; IL-1β, interleukin-1 beta

    Article Snippet: Equal amounts of protein (15 μg) were separated by electrophoresis on a 10% sodium dodecyl sulfate-polyacrylamide gel at 60 V for 1 h and 45 min, followed by 100 V for 1 h and 15 min. Proteins were then transferred to membranes and incubated overnight at 4 °C with the following primary antibodies: β-actin mouse monoclonal antibody (1:8000; K200058M, Solarbio Life Science, Beijing, China), PARP-1 mouse monoclonal antibody (1:500; SC-74470, Santa Cruz Biotechnology, Dallas, TX, USA), PAR/pADPr mouse monoclonal antibody (1:1000; 4335-MC-100, Bio-techne R&D Systems, Minneapolis, MN, USA), cleaved caspase-3 rabbit monoclonal antibody (1:1000; 9664, Cell Signaling Technology, Danvers, MA, USA), NOD-like receptor protein 3 (NLRP3) rabbit monoclonal antibody (1:1000; 15101, Cell Signaling Technology), caspase-1 rabbit monoclonal antibody (1:1000; 24232, Cell Signaling Technology), IL-1β rabbit monoclonal antibody (1:1000; 12703, Cell Signaling Technology), nuclear factor κB (NF-κB) p65 rabbit monoclonal antibody (1:1000; 8242, Cell Signaling Technology), phospho-nuclear factor κB (NF-κB) rabbit monoclonal antibody (1:1000; 3033, Cell Signaling Technology), C-Jun N-terminal protein kinase (JNK) mouse monoclonal antibody (1:500; SC-7345, Santa Cruz Biotechnology), and phospho-c-JNK mouse monoclonal antibody (1:500; SC-135642, Santa Cruz Biotechnology).

    Techniques: Inhibition, Cell Culture, MTT Assay, TUNEL Assay, Western Blot, Flow Cytometry, Annexin V Assay, Derivative Assay, Two Tailed Test, Knock-Out, Binding Assay

    (A) Through flow cytometry, the percentages of M1 macrophages (F4/80 + CD11b + CD86 + cells) and M2 macrophages (F4/80 + CD11b + CD206 + cells) in the livers of WT and ob/ob mice were determined. (B-D) Western blotting was employed to analyze CD206, CD86, CD80, NLRP3, and IL-1β in the liver of WT and ob/ob mice. The data are exhibited as the mean ± SEM in each group (n = 3-6).* p < 0.05, ** p < 0.01, n.s. not significant.

    Journal: PLOS One

    Article Title: Hyperuricemia in ob/ob mice relates to hepatocellular pyruvate metabolism/ xanthine oxidase axis

    doi: 10.1371/journal.pone.0328794

    Figure Lengend Snippet: (A) Through flow cytometry, the percentages of M1 macrophages (F4/80 + CD11b + CD86 + cells) and M2 macrophages (F4/80 + CD11b + CD206 + cells) in the livers of WT and ob/ob mice were determined. (B-D) Western blotting was employed to analyze CD206, CD86, CD80, NLRP3, and IL-1β in the liver of WT and ob/ob mice. The data are exhibited as the mean ± SEM in each group (n = 3-6).* p < 0.05, ** p < 0.01, n.s. not significant.

    Article Snippet: The membranes were blocked with TBST solution containing 5% non-fat milk at room temperature for 1 hour, then incubated overnight at 4 °C with primary antibodies: β-actin (#4970, CST, USA), CD86(13395–1-AP, Proteintech, China), Interleukin (IL)-1β (ab254360, Abcam, USA), Nod-like receptor protein 3 (NLRP3) (#15101, CST, USA), CD206(#24595, CST, USA), XOD(ab109235, Abcam, USA), CD80 (66406–1-Ig, Proteintech, China) and pyruvate dehydrogenase (PDH) (#2784, CST, USA).

    Techniques: Flow Cytometry, Western Blot

    (A) Immunoblot analysis of NLRP3, CD80 in THP-1 macrophages followed by palmitic acid (PA) treatment for 0–24 hours. (B) Schematic showing that HUH-7 cells were co-cultured with THP-1 macrophages with or without LPS + IFN-γ or PA. (C) Hepatic XOD activity in Huh-7 cells. The data are exhibited as the mean ± SEM in each group (n = 3-6). * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not significant.

    Journal: PLOS One

    Article Title: Hyperuricemia in ob/ob mice relates to hepatocellular pyruvate metabolism/ xanthine oxidase axis

    doi: 10.1371/journal.pone.0328794

    Figure Lengend Snippet: (A) Immunoblot analysis of NLRP3, CD80 in THP-1 macrophages followed by palmitic acid (PA) treatment for 0–24 hours. (B) Schematic showing that HUH-7 cells were co-cultured with THP-1 macrophages with or without LPS + IFN-γ or PA. (C) Hepatic XOD activity in Huh-7 cells. The data are exhibited as the mean ± SEM in each group (n = 3-6). * p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not significant.

    Article Snippet: The membranes were blocked with TBST solution containing 5% non-fat milk at room temperature for 1 hour, then incubated overnight at 4 °C with primary antibodies: β-actin (#4970, CST, USA), CD86(13395–1-AP, Proteintech, China), Interleukin (IL)-1β (ab254360, Abcam, USA), Nod-like receptor protein 3 (NLRP3) (#15101, CST, USA), CD206(#24595, CST, USA), XOD(ab109235, Abcam, USA), CD80 (66406–1-Ig, Proteintech, China) and pyruvate dehydrogenase (PDH) (#2784, CST, USA).

    Techniques: Western Blot, Cell Culture, Activity Assay